The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 18, 2000
Filed:
Jul. 31, 1998
Shi-Lung Lin, Alhambra, CA (US);
Shao-Yao Ying, San Marino, CA (US);
Epiclone Inc., Alhambra, CA (US);
Abstract
The present invention provides a simple, specific and nontoxic gene knock-out method by formation of covalently bonding between modified probes and targeted sequences. When covalently modified first strand probes are hybridized with a second strand of targeted gene transcripts, certain modified bases of said first strand will interact with natural bases of said second strand to form covalent bonds by which the translation of said second strand is inhibited. Because the hybridization of said two strands generates covalent base-pairing only between their complementary homology region(s), such specificity increases the targeting efficiency of a gene knock-out system. Also, because neither a polymerase extension reaction nor a nuclease digestion can be performed through the covalently bonded region(s) of aforesaid hybrids, the present invention in conjunction with a delivery method can be used to inactivate intracellular functions of targeted nucleotide sequences, to inhibit viral infections in vivo and to increase binding stability of antisense drugs in a gene therapy.