The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 07, 1999
Filed:
Jan. 13, 1999
Applicant:
Inventors:
Robert Mierendorf, Madison, WI (US);
Thomas R Van Oosbree, Madison, WI (US);
Assignee:
Novagen, Inc., Madison, WI (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N / ;
U.S. Cl.
CPC ...
435 681 ; 435 691 ; 435 701 ;
Abstract
A method for performing coupled in vitro transcription and translation reactions is disclosed. In the transcription reaction, the quantity of mRNA produced is limited to a level of less than about 2.5 micrograms in a 10 microliter volume prior to the translation elements being added. Limiting the level of mRNA produced prevents saturation of the translational processes and thus aids in the efficiency and fidelity of the translation process.