The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 09, 1999
Filed:
Feb. 12, 1997
Applicant:
Inventors:
David R Thatcher, Macclesfield, GB;
Anthony Hitchcock, Wistaston, GB;
Julian AJ. Hanak, Macclesfield, GB;
Diane L Varley, Willaston, GB;
Assignee:
Cobra Therapeutics Limited, Staffordshire, GB;
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
A61K / ; C07H / ; C07H / ; A23J / ;
U.S. Cl.
CPC ...
536 254 ; 424124 ; 435384 ; 435404 ; 536 2652 ; 536 2643 ; 71-8 ; 530417 ;
Abstract
A scalable method for the production of highly purified plasmid DNA in Escherichia coli is described, which method includes growing plasmid-containing cells to a high biomass in exponential growth and lysing the cells by raising the pH of the culture to a carefully controlled pH value in which chromosomal DNA is denatured but plasmid DNA is reversibly renatured. The method has been developed for the production of pharmaceutical grade DNA for use in in vivo and ex vivo gene therapy.