The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 20, 1999
Filed:
Jun. 07, 1995
Shaun R Coughlin, Tiburon, CA (US);
Ji Chen, San Francisco, CA (US);
Harold Bernstein, San Francisco, CA (US);
Maki Ishii, Kyoto, JP;
Ling Wang, San Francisco, CA (US);
Mian Chen, San Francisco, CA (US);
The Regents of the University of California, Oakland, CA (US);
Abstract
This invention relates to peptide ligand discovery and is particularly directed to a method for the discovery of agonists for membrane bound receptors. The inventive detection system involves the use of a 'tethered' ligand for probing receptor binding. The general detection system includes a membrane, a membrane bound receptor, and a chimeric ligand presenting molecule. This chimeric protein forms the tethered ligand and in turn includes a membrane domain, a linker domain, a ligand domain, and a cleavable terminal domain. The 'ligands' of the system are exposed by the addition of a specific peptidase that cleaves at the designated sequence. The sequence of the ligand that produces signal as a result of the interaction between the agonist and receptor can be then be isolated using sib selection.