The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 29, 1999
Filed:
May. 08, 1997
Applicant:
Inventors:
Robert E Pearson, Durham, NC (US);
Julie A Dickson, Raleigh, NC (US);
Majid Mehrpouyan, San Jose, CA (US);
Assignee:
Becton, Dickinson and Company, Franklin Lakes, NJ (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P / ; C07H / ;
U.S. Cl.
CPC ...
435 912 ; 435 911 ; 435-6 ; 536 2433 ;
Abstract
Strand Displacement Amplification has been adapted to reverse transcription amplification of RNA targets. The method of the invention is referred to as reverse transcription SDA (rtSDA) and may be performed as a two-step process or as a one-step process in which cDNA copies of an RNA target sequence are generated and amplified concurrently. In the one-step process, rtSDA reaction conditions are such that a conventional reverse transcriptase and a DNA-dependent polymerase function together in a single reaction to produce cDNA and amplify it.