The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 15, 1999

Filed:

Sep. 25, 1996
Applicant:
Inventors:

Juan Ruiz, Pamplona, ES;

George Y Wu, Avon, CT (US);

Assignee:

The University of Connecticut, Farmington, CT (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C07H / ; C07H / ; C12N / ; C12N / ;
U.S. Cl.
CPC ...
435 9131 ; 4353201 ; 435325 ; 435375 ; 536 231 ; 536 245 ; 536 251 ;
Abstract

The invention pertains to a multimeric self-cleaving ribozyme comprising multiple repeating units, wherein each unit comprises a ribozyme containing a catalytic domain and an antisense domain, and a target recognition sequence or portion thereof recognized by the antisense domain of the ribozyme and comprising a ribozyme cleavage site to facilitate self-cleavage. The invention also pertains to constructs comprising DNA encoding the multimeric self-cleaving ribozyme, cells stably transformed with these constructs, and methods of cleaving RNA, regulating gene expression and generating molecular weight ladders utilizing the multimeric self-cleaving ribozyme.


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