The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 16, 1999
Filed:
May. 10, 1996
Duane L Venton, Lombard, IL (US);
Charles P Woodbury, Villa Park, IL (US);
Richard B van Breemen, Elmhurst, IL (US);
Board of Trustees of the University of Illinois, Urbana, IL (US);
Abstract
A device and method is disclosed for the identification, concentration and isolation of a chemical compound from a mixture of compounds on the basis of the binding affinity of the chemical compound to a macromolecule or complex thereof, that is, to a receptor. Both the compounds to be assayed, and the macromolecules to which the compounds may bind, are free in solution. The method comprises contacting a mixture of compounds in solution with a solution of macromolecules or complexes thereof in a device possessing an ultrafiltration membrane to retain the receptor and bound compound receptor complex; washing through the device non-bound compounds; and releasing a bolus of the bound compound which passes through the ultrafiltration membrane where it may be analyzed chemically and/or spectrally (e.g. mass spectrometry) and/or bioassayed. The device includes a barrier such as an ultrafiltration membrane separating a binding chamber and an effluent chamber. An inlet port and wash port connect to the binding chamber. An effluent port connected to the effluent chamber is directed to analyzer equipment. Stirring means may be located within the binding chamber.