The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 08, 1998

Filed:

May. 30, 1995
Applicant:
Inventors:

Joseph A Akkara, Holliston, MA (US);

David L Kaplan, Stow, MA (US);

Madhu S Ayyagari, Brighton, MA (US);

Kenneth A Marx, Francestown, MA (US);

Sanjay Kamtekar, Lowell, MA (US);

Rajiv Pande, Lowell, MA (US);

Sukant K Tripathy, Acton, MA (US);

Jayant Kumar, Westford, MA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C07D / ; C12Q / ; C12Q / ;
U.S. Cl.
CPC ...
435 18 ; 549332 ; 549222 ; 435 19 ; 435 21 ;
Abstract

A method of detecting the presence of a substance being monitored in a medium, selected from the group of substances including organophosphorus compounds and the metal ions Zn, Be and Bi, including the steps of: providing a 1,2-dioxetane phenyl phosphate compound; providing a phosphatase that catalytically degrades the 1,2-dioxetane phenyl phosphate compound to produce light, the catalytic activity of the phosphatase toward 1,2-dioxetane phenyl phosphate compound being altered by the substance being monitored; exposing the 1,2-dioxetane phenyl phosphate compound and the phosphatase together to a medium which may contain the substance being monitored; detecting light produced after the exposing step; and determining, from the detected light, the presence and concentration in the medium of the substance being monitored.


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