The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 11, 1998

Filed:

Mar. 28, 1995
Applicant:
Inventors:

Keith C Backman, Bedford, MA (US);

Sheila B Bond, Lake Forest, IL (US);

John J Carrino, Gurnee, IL (US);

Thomas G Laffler, Libertyville, IL (US);

Assignee:

Abbott Laboratories, Abbott Park, IL (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ; C12P / ;
U.S. Cl.
CPC ...
435-6 ; 435 911 ; 435 912 ; 435810 ; 436501 ; 536 231 ; 536 241 ; 536 243 ; 536 2431 ; 536 2432 ; 536 2433 ; 935 77 ; 935 78 ; 935 88 ;
Abstract

The present invention involves methods of improving LCR and PCR amplification schemes by modifying at least one probe/primer end so that the probability of the probe/prirner contributing to spurious signal development is greatly reduced. Only after specific hybridization of the modified probe/primer with true target, are the modified ends 'corrected' in a target dependent fashion to allow participation of the probe/primer in the enzymatic assembly reaction. Specific modifications depend on whether the assembly is done by ligation (as in LCR) or by extension/elongation (as in PCR).


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