The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 04, 1998
Filed:
May. 11, 1995
Health Research Inc., Buffalo, NY (US);
Abstract
Methods and compositions are provided for use in electroloading procedures to increase the transfection and fusion efficiency compared to the methods now used in the art. The compositions comprise a two-phase polymer system containing two water soluble polymers which, when mixed, result in target cells and biological material being encapsulated into one of the polymer phases in a concentrated form. The methods of the present invention for electroloading biological material into target cells comprises mixing the biological material into one of the phases of the two-phase polymer system; mixing the target cells into either of the phases of the two-phase polymer system; mixing the phases together to form an emulsion; and exposing the emulsion to a pulsing electric field in an electroloading process.