The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 07, 1998

Filed:

May. 23, 1995
Applicant:
Inventors:

Fouad A El-Zaatari, Pearland, TX (US);

Saleh Naser, Orlando, FL (US);

David Y Graham, Houston, TX (US);

Assignee:

Baylor College of Medicine, Houston, TX (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C12P / ; C07H / ; C12N / ;
U.S. Cl.
CPC ...
435-6 ; 435 912 ; 4353201 ; 536 221 ;
Abstract

Recombinant clone pMptb #48 expressing a 36k M. paratuberculosis antigen is provided that provides a more sensitive and discriminating test than those in which crude antigens have been used. A mycobacterial genus-specific DNA probe corresponding to a 1.4 kb BamH1-DNA insert in pMptb #48 is also provided. The 1.4 kb insert includes the entire DNA coding sequence for an M. paratuberculosis 36k protein. There is also provided methods for serologically testing specimens which include the use of recombinant clone pMptb #48 and/or the p36k protein and/or fragments of the p36k protein. Also provided are methods for identifying mycobacterial infections using a probe corresponding to the 1.4 kb DNA sequence shown in FIG. 4, the coding portion of the 1.4 kb DNA sequence, or fragments thereof.


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