The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 17, 1998
Filed:
Mar. 29, 1996
Yukio Yamada, Tsushima, JP;
Osamu Asami, Konan, JP;
Hiroko Uchiyama, Nagoya, JP;
Hidehiko Sugiyama, Aichi-gun, JP;
Satoshi Fujita, Nisshin, JP;
Takekazu Yamamoto, Tokyo, JP;
Naoto Kagiyama, Sapporo, JP;
Masayoshi Momiyama, Sapporo, JP;
Yasumitsu Kondoh, Sapporo, JP;
Kabushiki Kaisha Toyota Chuo Kenkyusho, Aichi, JP;
Aisin Seiki Kabushiki Kaisha, Kariya, JP;
Abstract
A method of detecting nucleic acid utilizes hybridization between the nucleic acid to be detected which is present on an immobilizing support and a DNA probe which is complementary to at least a portion of the desired nucleotide sequence of the nucleic acid. The present DNA probe includes a hapten attached via a linker which a hapten is gibberellin or a gibberellin derivative. After hybridization by reaction between the nucleic acid to be detected and the DNA probe, the unreacted DNA probe is removed, and then labelled anti-hapten antibody is allowed to bind with the DNA probe of the hybrid and the labelling is used to detect the nucleic acid. Accordingly, stable detecting sensitivity may be achieved regardless of the label mixing ratio or labelling ratio, and thus regardless of the nucleotide sequence composition of the nucleic acid being tested.