The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 27, 1998

Filed:

Feb. 22, 1996
Applicant:
Inventors:

William S Adney, Golden, CO (US);

Steven R Thomas, Denver, CO (US);

John O Baker, Golden, CO (US);

Michael E Himmel, Littleton, CO (US);

Yat-Chen Chou, Wheat Ridge, CO (US);

Assignee:

Midwest Research Institute, Kansas City, MO (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12P / ; C17H / ;
U.S. Cl.
CPC ...
435209 ; 435 691 ; 4352523 ; 43525233 ; 4353201 ; 536 231 ; 536 232 ; 536 237 ;
Abstract

The gene encoding Acidothermus cellulolyticus E1 endoglucanase is cloned and expressed in Pichia pastoris. A new modified E1 endoglucanase enzyme comprising the catalytic domain of the full size E1 enzyme demonstrates enhanced thermostability and is produced by two methods. The first method of producing the new modified E1 is proteolytic cleavage to remove the cellulose binding domain and linker peptide of the full size E1. The second method of producing the new modified E1 is genetic truncation of the gene encoding the full size E1 so that the catalytic domain is expressed in the expression product.


Find Patent Forward Citations

Loading…