The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 22, 1997
Filed:
Jun. 07, 1995
Nico Cerletti, Bottmingen, CH;
Gary Kent McMaster, Kaiseraugst, CH;
David Cox, Himmelried, CH;
Albert Schmitz, Basel, CH;
Bernd Meyhack, Magden, CH;
Ciba-Geigy Corporation, Tarrytown, NY (US);
Abstract
The process for the production of a dimeric, biologically active Transforming Growth Factor type .beta. (TGF-.beta.)-like protein or a salt thereof, comprising refolding the denatured monomeric form of the protein in the presence of a mild detergent that permits folding of the monomeric protein into a spatial conformation, which after dimerization, is the conformation associated with the biological activity, while retaining the monomeric protein in soluble form. Mild detergents include sulfobetaines, 3-(3-chlolamidopropyl)dimethylammonio-1-propanesulfonate, 3-(3-chlolamidopropyl)dimethylammonio-2-hydroxy-1-propane-sulfonate, digitonin, cholate and deoxycholate at a concentration of about 1 to 100 mM. Compositions containing a TGF-.beta.-like protein produced according the invention can be used for the promotion and acceleration of wound healing and bone and tissue repair, cancer treatment, bone marrow protective agent, as a mediator of cardio-protection, as an anti-inflammatory or immunosuppressive agent, or as a growth regulator in mammalian cell cultures.