The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 22, 1997
Filed:
May. 15, 1996
Sek Wen Hui, Williamsville, NY (US);
Li Lin-Hong, Buffalo, NY (US);
Mary L Hensen, East Aurora, NY (US);
Ya Li Zhao, Buffalo, NY (US);
Health Research Inc., Buffalo, NY (US);
Abstract
A method and apparatus for electrofusing a cell pellet comprising a plurality of tiers of cells of two different cell types to be fused which results in the formation of hybrid cells with high efficiency and high viability. The method comprises subjecting the cell pellet, and a pulse medium in contact with the cell pellet, with multiple pulses of voltage. A method and apparatus for electrofusing a cell-vesicle pellet comprising a plurality of tiers of a cell type and of membrane vesicles, and wherein the membrane vesicles are loaded with an exogenous macromolecule to be delivered to the cell type during fusion, which results in the formation of fused cells containing the exogenous macromolecule with high efficiency and high viability. Alteratively, the membrane vesicles may contain at least one type of exogenous macromolecule inserted into its membrane. Fusion with a target cell results in the exogenous macromolecule being incorporated into the cell membrane of the target cell with high efficiency and high viability. The method comprises subjecting the cell-vesicle pellet, and a pulse medium in contact with the cell-vesicle pellet, with multiple pulses of voltage.