The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 17, 1997
Filed:
Nov. 09, 1994
Applicant:
Inventors:
R Bruce Wallace, Greenbrae, CA (US);
Bijay K Pal, Arcadia, CA (US);
Luis A Ugozzoli, San Rafael, CA (US);
Dan Y Wu, Bellevue, WA (US);
Assignee:
City of Hope, Duarte, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P / ; C12Q / ;
U.S. Cl.
CPC ...
435-6 ; 435 912 ; 935 77 ; 935 78 ;
Abstract
A rapid, non-radioactive approach to the diagnosis of sickle cell anemia is described based on an allele specific polymerase chain reaction (ASPCR) in which the 3'-terminal nucleotide of one of the primers of the primer set forms a match with one allele and a mismatch with the other allele. This method allows the direct detection of the normal or the sickle cell .beta.-globin allele in genomic DNA without the additional steps of probe hybridization, ligation or restriction enzyme cleavage.