The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 06, 1997
Filed:
Oct. 27, 1994
Applicant:
Inventors:
PoJen Shih, Columbia, MD (US);
Pamela Hawley-Nelson, Silver Spring, MD (US);
Joel A Jessee, Mt. Airy, MD (US);
Assignee:
Life Technologies, Inc., Gaithersburg, MD (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K / ; A61K / ; C12N / ;
U.S. Cl.
CPC ...
514 44 ; 424450 ; 4351721 ; 4351723 ; 935 52 ; 935 54 ;
Abstract
A method is provided for enhancing transfection efficiency of eukaryotic cells comprising contacting said cells with a lipid aggregate comprising nucleic acid and a polycationic lipid composition in the presence of a polycationic compound and serum. The polycationic compound is preferably Polybrene.TM., and the lipid aggregate preferably comprises liposomes of nucleic acid and LipofectAMINE.TM..