The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 04, 1997

Filed:

Dec. 22, 1994
Applicant:
Inventors:

Dennis Fenton, Newbury Park, CA (US);

Por-Hsiung Lai, Westlake Village, CA (US);

Hsieng Lu, Thousand Oaks, CA (US);

Michael Mann, Thousand Oaks, CA (US);

Larry Tsai, Thousand Oaks, CA (US);

Assignee:

Amgen Inc., Thousand Oaks, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P / ; C12P / ;
U.S. Cl.
CPC ...
435 691 ; 435 695 ; 435 6951 ; 435 6952 ;
Abstract

Norleucine incorporation into recombinant gene products may be inhibited by culturing the cells expressing the gene products in media comprising a high level of leucine and/or methionine, or by expressing the gene products in a leu mutant. Norleucine incorporation in recombinant gene products may be promoted by culturing the cells expressing the gene products in media comprising norleucine or deficient in leucine and/or methionine. For example, norleucine analogs of IL-2, GCSF, .gamma.-interferon and .alpha.-consensus interferon may be produced in this way.


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