The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 15, 1996
Filed:
Jan. 27, 1995
Alex Chenchik, Palo Alto, CA (US);
Luda Diatchenko, Palo Alto, CA (US);
Paul Siebert, Sunnyvale, CA (US);
Sergey Lukianov, Moscow, SU;
Konstantin Lukianov, Moscow, SU;
Nadia Gurskaya, Moscow, SU;
Victor Tarabykin, Moscow, SU;
Eugene Sverdlov, Moscow, SU;
Clontech Laboratories, Inc., Palo Alto, CA (US);
Abstract
The subject invention pertains to novel materials and methods for suppressing amplification of particular DNA fragments during polymerase chain reaction (PCR). The PCR suppression method uses novel adapters that are ligated to the end of a DNA fragment prior to PCR amplification. Upon melting and annealing, single-stranded DNA fragments having self-complementary adapters at the 5'- and 3'-ends of the strand can form suppressive 'pan-like' double-stranded structures that suppress amplification of the fragments during PCR. The subject method offers improved specificity and sensitivity of PCR amplification of a target DNA and does not require target DNA sequence information. The subject invention can be adapted to a variety of highly useful PCR techniques and applications.