The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 15, 1996
Filed:
Dec. 27, 1995
Applicant:
Inventors:
Assignee:
Dade International Inc., Deerfield, IL (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; H01L / ;
U.S. Cl.
CPC ...
435 25 ; 435 18 ; 435 29 ; 435 34 ; 435 37 ; 435968 ; 436172 ;
Abstract
The present invention relates to a method to determine the concentration of an unknown substance in a colorimetric reaction using a fluorometric reader. The change in color can be monitored by observing the change of fluorescence of a known amount of a suitable fluorophore in the reaction chamber. Under appropriate conditions the absorption spectrum of the chromophore overlaps with the emission spectrum of the fluorophore thereby allowing the change in fluorescence to be proportional to the intensity of color in the reaction and thus, proportional to the quantity of the substance of interest. Specifically, 8-methoxypyrene tri-sulfonic acid is used as the fluorophore.