The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 01, 1996
Filed:
May. 24, 1995
Applicant:
Inventors:
David H Gelfand, Oakland, CA (US);
Thomas W Myers, Alameda, CA (US);
Christopher L Sigua, Antioch, CA (US);
Assignee:
Hoffmann-La Roche Inc., Nutley, NJ (US);
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P / ; C12Q / ;
U.S. Cl.
CPC ...
435912 ; 435-6 ; 435 911 ; 435 9121 ; 435 9151 ; 935 77 ; 935 78 ;
Abstract
Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. In a preferred embodiment, high temperature reverse transcription is coupled to nucleic acid amplification in a one tube, one enzyme procedure using a thermostable DNA polymerase. Methods for eliminating carry over contamination of amplifications due to prior reverse transcription reactions are also provided. Reagents particularly suited for the methods of the present invention are provided.