The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 02, 1996
Filed:
Jun. 20, 1994
Applicant:
Inventors:
George D Cimino, Richmond, CA (US);
Stephen T Isaacs, Orinda, CA (US);
John W Tessman, San Francisco, CA (US);
Assignee:
HRI Research, Inc., Concord, CA (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P / ; C07H / ;
U.S. Cl.
CPC ...
435 912 ; 435-6 ; 435 911 ; 435810 ; 536 221 ; 536 231 ; 536 253 ; 935 77 ; 935 78 ; 935 88 ;
Abstract
Methods for sterilization of ligase-based amplification products, including products of the Ligation Amplification Reaction (LAR), involving treatment of nucleic acid after amplification to render such nucleic acid incapable of serving as a template for further amplification. Both random and site-specific addition of sterilizing compounds, and more specifically, photoactivation compounds, render amplification product subsequently unamplifiable. Self-sterilizing primers are also employed allowing for a homogenous detection of ligase-based amplification product.