The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 02, 1996
Filed:
Mar. 04, 1992
Yoshie Kurihara, Setagaya-ku, Tokyo 116, JP;
Soichi Arai, Kanagawa-Ku,Yokohama-shi, Kanagawa-ken 221, JP;
Keiko Abe, Warabi, JP;
Haruyuki Yamashita, Arakawa, JP;
Other;
Asahi Denka Kogyo K.K., Tokyo, JP;
Abstract
Essentially pure curculin B is disclosed. A process for producing curculin B, includes the steps of: culturing a transformed cell or microorganism containing a recombinant DNA containing a base sequence encoding curculin B, whereby curculin B is produced by the transformed cell or microorganism, and isolating curculin B from the transformed cell or microorganism. A DNA encoding mature or premature curculin B is also disclosed. DNA including a base sequence encoding curculin B, is produced by: separating a fraction containing a curculin B mRNA from Curculigo latifolia, preparing a single-stranded DNA from the mRNA using reverse transcriptase, preparing a double-stranded DNA from the single-stranded DNA, inserting the double-stranded DNA into a vector, transforming a host with the vector to produce a cDNA library, and isolating a cDNA encoding curculin B from the library, using one or more synthesized DNA's containing a base sequence coding for a partial amino acid sequence elucidated from curculin A purified from Curculigo latifolia, as a probe.