The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 21, 1995
Filed:
Sep. 08, 1994
Applicant:
Inventors:
Victoria L Boyd, San Carlos, CA (US);
MeriLisa Bozzini, Burlingame, CA (US);
G Marc Loudon, West Lafayette, IN (US);
Assignee:
Perkin-Elmer, Norwalk, CT (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K / ; G01N / ;
U.S. Cl.
CPC ...
530345 ; 436 89 ; 436 90 ; 530408 ;
Abstract
A method is provided for C-terminal sequencing of a protein or peptide. An important feature of the method is the formation of an oxazolone moiety at the C-terminus of a protein or peptide by treatment with acetic anhydride under basic conditions followed by conversion of the oxazolone to a thiohydantoin moiety by treatment with thiocyanate under acidic conditions. Yields of thiohydantoin are further enhanced by delivering thiocyanate as the conjugate acid of a sterically hindered alkylammnonium cation.