The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 29, 1995

Filed:

Oct. 20, 1994
Applicant:
Inventors:

Frans Koster, Delft, NL;

Jos J Webbers, Maassluis, NL;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12P / ;
U.S. Cl.
CPC ...
435 711 ; 435 712 ; 435 713 ; 435183 ; 435187 ; 435198 ; 435272 ; 435800 ; 435814 ; 435815 ; 435816 ; 435874 ;
Abstract

A method of separating a hydrophobic fermentation product selected from the group consisting of lipase, esterase, endoxylanase and an antibiotic from a mixture comprising said product and contaminants which method comprises adding to the mixture sequentially (1) 0.5 to 15% (w/v) of a nonionic surfactant, (2) 0.5 to 60 mg of a flocculating agent per gram of said mixture, (3) 1 to 20% (w/v) of an extra nonionic surfactant, (4) a suitable K, Na, NH.sub.4 or Mg salt selected from the group of chlorides, sulfates, acetates, carbonates or phosphates, whereby the concentration of the salt is chosen so as to have the surfactant layer on top and is between 2 and 30%; to obtain a three phase product mixture, separating the product mixture into liquid-liquid-solid fractions and recovering the hydrophobic fermentation product.


Find Patent Forward Citations

Loading…