The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 25, 1995

Filed:

Jul. 12, 1993
Applicant:
Inventors:

Richard P Haugland, Eugene, OR (US);

Stephen T Yue, Eugene, OR (US);

Paul J Millard, Eugene, OR (US);

Bruce L Roth, Corvallis, OR (US);

Assignee:

Molecular Probes, Inc., Eugene, OR (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C12Q / ; G01N / ; C07H / ;
U.S. Cl.
CPC ...
435 34 ; 435-6 ; 435 29 ; 435 39 ; 435-4 ; 436 94 ; 436800 ; 536 2673 ; 536-111 ; 536 256 ;
Abstract

The invention describes the preparation and use of fluorescent stains for nucleic acids derived from unsymmetrical cyanine dyes. In particular, the invention describes unsymmetrical cyanine dyes having a saturated or unsaturated cyclic substituent. The dyes of the invention possess superior fluorescent characteristics when complexed with nucleic acids, and have utility in any application which requires detection of nucleic acids. The presence of the cyclic substituent results in improved permeability in a wide range of living cells, resulting in improved detection of intracellular nucleic acids.

Published as:

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