The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 11, 1995
Filed:
Mar. 22, 1994
Applicant:
Inventors:
Toshio Ichikawa, Noda, JP;
Yasuji Koyama, Noda, JP;
Hideko Otake, Noda, JP;
Eiichi Nakano, Noda, JP;
Assignee:
Kikkoman Corporation, Noda, JP;
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12N / ;
U.S. Cl.
CPC ...
435190 ; 435 691 ; 435189 ; 43525233 ; 4353201 ; 536 232 ; 935 10 ; 935 14 ; 935 29 ; 935 73 ;
Abstract
The present invention relates to variant E.sub.1 protein of pyruvate dehydrogenase complex of high activity in which arginine at the 146-position is replaced by proline in the amino acid sequence of wild-type E.sub.1 protein of pyruvate dehydrogenase complex, a gene coding for said variant E.sub.1 protein, a novel recombinant DNA comprising said variant E.sub.1 protein-encoding gene inserted in a vector DNA, and a process for producing variant E.sub.1 protein by said recombinant DNA. The present invention provides the variant E.sub.1 protein of pyruvate dehydrogenase complex of high activity.