The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 27, 1995
Filed:
Jun. 28, 1991
Larry G Birkenmeyer, Chicago, IL (US);
John J Carrino, Gurnee, IL (US);
Bruce J Dille, Antioch, IL (US);
Hsiang-Yun Hu, Libertyville, IL (US);
Jon D Kratochvil, Kenosha, WI (US);
Thomas G Laffler, Libertyville, IL (US);
Ronald L Marshall, Zion, IL (US);
Laurie A Rinehardt, Kenosha, WI (US);
Natalie A Solomon, Buffalo Grove, IL (US);
Abbott Laboratories, Abbott Park, IL (US);
Abstract
An improved, 'gap filling' embodiment of the Ligase Chain Reaction (LCR) is described. Gap filling LCR is LCR wherein at least one of the probes is recessed so that a gap is formed between the adjacent probes when they are hybridized to target. The gap is filled using polymerase and deoxyribonucleotide triphosphates before ligation of the probes together. There are single and double gap versions, depending on whether one or two probes are recessed and require filling before ligation. The improvement resides in selecting and using target sequences such that only a single type, or two types, of deoxyribonucleotide triphosphate(s) are required to fill double gaps each being 1-10 bases in length, preferably 1-3 bases. Probes having specific sequences are claimed for a number of pathogens.