The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 24, 1995
Filed:
Aug. 26, 1993
Anne-Marie Lambeir, Heverlee, BE;
Ignace Lasters, Antwerp, BE;
Nadir Mrabet, Hoeilaart, BE;
Wilhelmus J Quax, Voorschoten, NL;
Jan M Van der Laan, Groningen, NL;
Onno Misset, Delft, NL;
Gist-brocades, N.V., Delft, NL;
Plant Genetics System, NV, Brussels, BE;
Abstract
A method for selecting amino acid residues is disclosed which upon replacement will give rise to an enzyme with an altered pH optimum. The method is specific for metalloenzymes which are inactivated at low pH due to the dissociation of the metal ions. The method is based on altering the pK.sub.a of the metal coordinating ligands or altering the K.sub.ass for the metal binding. New glucose isomerases with an altered pH optimum are provided according to this method. These altered properties enable starch degradation to be performed at lower pH values.