The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 02, 1994
Filed:
Apr. 01, 1993
Applicant:
Inventors:
Trevor H Adams, Woodinville, WA (US);
Dennis E Schwartz, Redmond, WA (US);
Nicolaas M Vermuelen, Woodinville, WA (US);
Roy H Kanemoto, Seattle, WA (US);
Assignee:
Microprobe Corporation, Bothell, WA (US);
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ; C07H / ; C07H / ; C12N / ;
U.S. Cl.
CPC ...
435-6 ; 536 231 ; 536 2432 ; 935 77 ; 935 78 ;
Abstract
This invention provides for a method of quantifying bacteria using a bacterial specific nucleic acid probe which is complementary to a unique and highly conserved region of the 16S ribosomal RNA (rRNA) of bacteria. This probe permits the rapid detection of 16S rRNA in a sample and by comparison with known standards, one can estimate the total bacterial count in the sample. The method is accurate and reproducible and conducted at temperatures of between about 120.degree. to about 40.degree. C.