The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 14, 1994
Filed:
Aug. 26, 1992
Applicant:
Inventors:
Morita Iwami, Tsukuba, JP;
Ichiro Aramori, Kyoto, JP;
Masao Fukagawa, Tsuchiura, JP;
Takao Isogai, Ibaraki, JP;
Hitoshi Kojo, Tsuchiura, JP;
Assignee:
Fujisawa Pharmaceutical Co., Ltd., Osaka, JP;
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P / ; C12P / ;
U.S. Cl.
CPC ...
435 47 ; 536 232 ; 435 691 ; 435 712 ; 4352523 ; 43525233 ; 4353201 ; 4351723 ; 935 14 ; 935 29 ; 935 56 ; 935 72 ; 935 73 ;
Abstract
A cephalosporin C acylase from Pseudomonas diminuta N-176 is characterized by its ability to catalyze the conversion of cephalosporin C, glutaryl 7-ACA, adipyl 7-ACA, succinyl 7-ACA, N-acetylcephalosporin, N-benzoylcephalosporin C and cephalothin into 7-aminocephalosporanic acid. The enzyme contains an .alpha.-subunit having a molecular weight of 26 kDA and a .beta.-subunit having a molecular weight of 58 kDa. A method for the recombinant production of the present cephalosporin C acylase in Escherichia coli is provided.