The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 18, 1994
Filed:
May. 15, 1991
Paolo Fabrizi, Monteriggioni, IT;
Francesco Donnini, Arezzo, IT;
Alessandro Tabacco, Monteriggioni, IT;
Paolo Tarli, deceased, late of Monteriggioni, IT;
Sclavo S.p.A., Siena, IT;
Abstract
The enzyme alanine aminotransferase (ALT) is colorimetrically determined as the hydrogen peroxide obtained in the pyruvate hydrolysis reaction catalysed by the enzyme pyruvate oxidase which develops a colour read at 550 nm making use of a reagent composition buffered at pH comprised within 7.0 and 7.5 containing L-alanine, ketoglutaric acid, a source of inorganic phosphorous, the enzyme pyruvate oxidase, a system for revealing the hydrogen peroxide and optionally one or more co-factors which interact in the enzymatic reaction catalysed by the enzyme pyruvate oxidase. It is moreover described a method for the determination of the enzyme alanine aminotransferase making use of said reagent composition and of the surface antigen of the hepatitis B virus (HBsAg), by enzyme-immune assay, in the same serum specimen and in the same well of a microtitration plate. The method is particularly useful for checking the suitability of the blood intended for transfusions since it can be easily carried out with equipments and instruments widely used for the immunoenzymatic screening of infectivity. Furthermore it enables to test in the same analytical assay the two labels of hepatocyte infections necessarily researched in the transfusion centres on biological specimens and to select among the donors the most likely candidates to transmission of the NANB hepatitis virus.