The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 20, 1993
Filed:
Oct. 06, 1988
Applicant:
Inventors:
Michael C Deeley, Seattle, WA (US);
Steven D Gimpel, Seattle, WA (US);
Virginia L Price, Seattle, WA (US);
Assignee:
Immunex Corporation, Seattle, WA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K / ;
U.S. Cl.
CPC ...
530351 ; 530395 ; 435691 ; 435 695 ; 435 699 ; 930145 ;
Abstract
Amplified expression of recombinant DNA products is achieved in hosts expressing protease that cleave at multi-basic amino acid residues. To this end, wild-type genes encoding the desired protein products are mutated by substituting codons or eliminating codons encoding multi-basic amino acid residues while maintaining the activity of the expressed protein product. Mutation of the desired gene can be conveniently carried out by site-specific in vitro mutagenisis.