The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 22, 1993
Filed:
Dec. 05, 1989
Applicant:
Inventors:
Anthony F Purchio, Seattle, WA (US);
Linda Madisen, Seattle, WA (US);
Nancy Webb, College Station, TX (US);
Assignee:
Oncogen, Seattle, WA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P / ; C12P / ; C12N / ; C12N / ; C12N / ; C07K / ;
U.S. Cl.
CPC ...
435 697 ; 455 691 ; 455 695 ; 455 91 ; 4551723 ; 4552351 ; 4552402 ; 530350 ; 935 19 ; 935 32 ; 935 34 ; 935 47 ; 935 57 ; 935 62 ; 935 70 ;
Abstract
cDNA clones coding for TGF-.beta.2 which are used to construct expression vectors capable of directing the high-level expression of mature, biologically active TGF-.beta.2, as well as precursor TGF-.beta.2 forms, in transfected Chinese Hamster Ovary cells (CHO cells) and transfected COS cells are described. CHO and COS transfectants secreting TGF-.beta.2 at high levels are also described. CHO cells transfected with a plasmid vector carrying the complete 414 amino acid simian TGF-.beta.2 precursor secrete approximately 5 .mu.g per ml culture media.