The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 09, 1993
Filed:
Aug. 20, 1991
Applicant:
Inventors:
Morita Iwami, Tsukuba, JP;
Ichiro Aramori, Kyoto, JP;
Masao Fukagawa, Tsuchiura, JP;
Takao Isogai, Ibaraki, JP;
Hitoshi Kojo, Tsuchiura, JP;
Assignee:
Fujisawa Pharmaceutical Co., Ltd., Osaka, JP;
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ;
U.S. Cl.
CPC ...
435228 ; 435 691 ; 435 51 ; 4351723 ; 4352523 ; 43525233 ; 4353201 ; 935 14 ; 935 29 ; 935 56 ; 935 73 ; 536 232 ;
Abstract
A cephalosporin C acylase from Pseudomonas diminuta N-176 is disclosed as well as the recombinant production of this enzyme in E. coli. The enzyme is characterized by the ability to catalyze the conversion of cephalosporin C, gultaryl 7-ACA, apidyl 7-ACA, succinyl 7-ACA, N-acetylcephalosporin C, N-benzoylcephalosporin C, and cephalothin into 7-aminocephalosporanic acid and is composed of an .alpha.-subunit with a molecular weight of 26,000 daltons and a .beta.-subunit with a molecular weight of 58,000 daltons.