The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 05, 1992
Filed:
Jul. 31, 1989
Applicant:
Inventors:
Yoshinari Myoken, Lake Placid, NY (US);
Tetsuji Okamoto, Hiroshima, JP;
J Denry Sato, Lake Placid, NY (US);
Assignee:
W. Alton Jones Cell Science Center Incorporated, Lake Placid, NY (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ;
U.S. Cl.
CPC ...
43524027 ; 43524031 ; 4352402 ; 435 702 ; 4352403 ; 43524021 ;
Abstract
Cholesterol auxotrophy of myeloma cells is used as the basis for selecting hybridomas. The outgrowth of nascent hybridomas in a cholesterol-free medium was 3- to 9-fold more efficient than that in HAT medium and resulted in 3- to 13-times as many antigen-reactive hybridoma cells. This method of hybridoma selection can be applied with any sterol-dependent parent cell line. The nutrient medium is also preferably free of Ham's F-12 nutrient mixture.