The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 18, 1992

Filed:

Jun. 14, 1989
Applicant:
Inventors:

Peter J Kushner, San Francisco, CA (US);

Claire L Cofer, Fremont, CA (US);

Jeffrey S Friedman, Palo Alto, CA (US);

Karen D Talmadge, Palo Alto, CA (US);

Assignee:

California Biotechnology Inc., Mountain View, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P / ; C12N / ; C12N / ; C12N / ;
U.S. Cl.
CPC ...
435 691 ; 4351723 ; 4352402 ; 4353201 ; 4352401 ; 536 27 ; 935 32 ; 935 34 ; 935 70 ;
Abstract

An expression system for recombinant production of a desired protein comprises CHO cells transformed with a DNA sequence having the desired protein coding sequence under control of the human metallothionein-II promoter. The cells can be maintained on serum-free medium and induced in the presence of an induction mediator. In addition, the system may include an enhancer element and/or a resistance-conferring gene to provide increased levels of expression. The system can process genomic as well as intronless DNA, and is capable of producing proteins which have the same characteristics as those obtained from native sources. Human growth hormone which is indistinguishable from that produced by the pituitary has been thus produced.


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