The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 10, 1991
Filed:
Dec. 22, 1988
Applicant:
Inventors:
Guido Grandi, Segrate, IT;
Elisabetta Franchi, Milan, IT;
Federico Maisano, Lodi, IT;
Silvia Astrua Testori, Milan, IT;
Assignee:
Eniricerche S.p.A., Milan, IT;
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P / ; C12P / ; C12N / ; C07K / ;
U.S. Cl.
CPC ...
435 681 ; 435 694 ; 435 697 ; 43525231 ; 530399 ; 935 47 ; 935 48 ; 935 74 ;
Abstract
An improved method is described for the preparation of natural human growth hormone (hGH) in pure form, which comprises the preparation of a precursor of hGH by means of the culture of Bacillus subtilis cells transformed by a hybrid plasmid, the separation of the precursor from the total proteins, the digestion of the precursor with the enzyme Factor Xa and finally the purification of the natural hGH thus obtained from the enzymatic hydrolysis mixture.