The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 07, 1991
Filed:
Feb. 12, 1990
Eiko Ohtsuka, Sappro, JP;
Hideo Inoue, Sappro, JP;
Hirokazu Morisawa, Kawasaki, JP;
Susumu Shibahara, Kawasaki, JP;
Sachiko Mukai, Kawasaki, JP;
Tohru Nishihara, Kurashiki, JP;
Ajinomoto Co., Inc., Tokyo, JP;
Abstract
There is disclosed a compound having a double chain which is composed of an RNA (+chain) and a complementary DNA (-chain), wherein a portion of the DNA (-chain) has been replaced by an RNA or a derivative thereof, and wherein, when the compound is subjected to the action of an enzyme having a ribonuclease H activity, it is possible to preferentially cleave the RNA (+chain) in a position corresponding to the unsubstituted portion of the DNA (-chain). The compound can thus be used for the preferential cleavage of a phosphodiester bond in a specific position of RNA. Accordingly, the invention provides a useful means for preparing, for instance, a deletion mutant. There is also disclosed a mixed oligomer which comprises an oligomer of RNA or a derivative thereof and a DNA oligomer, wherein the RNA oligomer or a derivative thereof is conjugated to the DNA oligomer via a phosphate diester linkage between the 5'-hydroxyl group and the 3'-hydroxyl group in the ribose or deoxyribose moiety. There is further disclosed a nucleoside derivative of a given general formula for use as a starting material.