The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 08, 1991
Filed:
Oct. 26, 1988
Pierre F Lardinois, Louvain-la-Neuve, BE;
Paul G Rouxhet, Louvain-la-Neuve, BE;
William E Stone, Louvain-la-Neuve, BE;
Kenneth M Baker, Brussels, BE;
Alain E Baudichau, Tourinnes-la-Grosse, BE;
Monsanto Company, St. Louis, MO (US);
Abstract
Enzymes such as L-methionine gamma lyase, L-lysine decarboxylase, L-aspartase and L-tryptophanase are immobilized by contacting with a latex of polymer particles having a negative surface charge such that the electrophoretic mobility of the latex has a negative value of -2.0 10.sup.-8 m.sup.2 V.sup.-1 sec.sup.-1 or below when measured at pH 7 in 0.01M KNO.sub.3 and a polymer solids concentration of 30 mg/l. The polymer particles also have a hydrophobic surface such that the contact angle of a 1 microliter droplet of distilled water with a plane horizontal surface formed by drying and compacting the latex particles is 70.degree. or more. The resultant-enzyme/polymer particles can be used for assaying amino acids corresponding to the specific enzyme immobilized in aqueous solutions using an electrosensor comprising a pH-sensitive electrode located in a sensing zone or chamber containing the enzyme/polymer particles as an aqueous dispersion or in water-dispersible form.