The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 08, 1990
Filed:
Sep. 26, 1988
Applicant:
Inventors:
Jesse L Bobbitt, Indianapolis, IN (US);
Joseph Manetta, Indianapolis, IN (US);
Assignee:
Eli Lilly and Company, Indianapolis, IN (US);
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C07K / ; C07K / ; C07K / ; C07K / ;
U.S. Cl.
CPC ...
530351 ; 530350 ; 530399 ; 530303 ; 530418 ; 530420 ; 530427 ; 435 6952 ;
Abstract
The present invention discloses a new method for solubilizing and refolding recombinant proteins expressed as granules. The method involves sulfitolysis and the formation of a precipitate of protein-S-sulfonate by warming. The precipitate has been found to contain protein in high purity. In addition, proper folding takes place if the desired protein is fully reduced and passed through an intermediate concentration of denaturant which allows for a transition between its folded and unfolded states.