The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 19, 1989

Filed:

Jan. 20, 1987
Applicant:
Inventors:

Charles M Radding, Hamden, CT (US);

Saul M Honigberg, New Haven, CT (US);

Sherman Weissman, New Haven, CT (US);

Basil Rigas, New York, NY (US);

Andrew A Welcher, New Haven, CT (US);

David C Ward, Guilford, CT (US);

Assignee:

Yale University, New Haven, CT (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C07H / ; C12N / ;
U.S. Cl.
CPC ...
435-6 ; 435803 ; 435810 ; 4351723 ; 935 78 ; 935 28 ; 536 27 ; 436808 ;
Abstract

A stable, single-stranded nucleoprotein filament adapted to complex specifically and stably with a target duplex DNA having a selected base sequence. The filament is composed of a single-stranded DNA probe having a region of homology with the target base sequence, and RecA protein bound stably to the DNA probe by adenosine 5'-(.gamma.-thio)triphosphate. The filament is useful in a novel system and method for enriching target duplex DNA which contains a region homologous to the probe sequence, for blocking selected restriction endonuclease sites in the target DNA, and in other DNA methodologies in which stable rapid triple-strand synaptic formation in duplex DNA can be exploited.


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