The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 12, 1989

Filed:

Mar. 11, 1987
Applicant:
Inventors:

Junichiro Asaka, Sakai, JP;

Tamio Fujiwara, Amagasaki, JP;

Takashi Fujiwara, Nara, JP;

Goro Kuwajima, Nishinomiya, JP;

Eiji Kondo, Ikeda, JP;

Masaru Shin, Kobe, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P / ; C12P / ; C12N / ; C12N / ;
U.S. Cl.
CPC ...
435 697 ; 4351723 ; 43525233 ; 435320 ; 536 27 ; 935 11 ; 935 29 ; 935 39 ; 935 44 ; 935 56 ; 935 60 ; 935 73 ;
Abstract

The whole DNA sequence coding for flagellin of E. coli (hag gene) cloned in phage .lambda. was determined. The hag gene is introduced into pBR322 and the region of the hag gene concerning the antigencity of flagella is lacked, into which linkers are inserted. Where the vector in which foreign DNA is inserted in the linker is introduced in E. coli forming no flagella, the E. coli can form flagella and possesses motility. The flagella comprises flagellin fused with foreign peptide encoded by the foreign DNA. Namely, the foreign peptide is excreted outside of bacteria. This system can be utilized in determination of epitope and preparation of antigen as well as excretion of peptide.


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