The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 24, 1989
Filed:
Nov. 18, 1987
Hiromi Ishiwa, Kodaira, JP;
Harue Shibahara, Nishitama, JP;
Masahiko Mutai, Higashiyamato, JP;
Nobuo Tsuchida, Yokohama, JP;
Kabushiki Kaisha Yakult Honsha, Tokyo, JP;
Abstract
A variety of chimeric plasmids each of which comprises (a) a tetracycline resistance gene (Tc) deriving from pAM.alpha.1, one of the plasmids retained by Streptococcus faecalis, (b) an ampicillin resistance gene (Amp) deriving from pACYC177, one of the vectors applicable to Escherichia coli and (c) either or both of origins, an origin (OripAM.alpha.1) deriving from the plasmid pAM.alpha.1 and an origin (Ori177) deriving from the vector pACYC177, the tetracycline resistance gene existing on each of which has the unique cleavage site for the restriction enzyme BalI on the entire DNA of the same plasmid and the ampicillin resistance gene existing on each of which has the unique cleavage sites for respective restriction enzymes BglI and PstI on the entire DNA on the same plasmid. There is also provided a chimeric plasmid vector containing (a) a tetracycline resistance gene region (Tc) derived from the plasmid pAM.alpha.1 of Streptococcus faecalis DS5 (ATCC14508), (b) an ampicillin resistance gene region (Amp) derived from the vector pACYC177 of E. coli, (c) a first DNA replication origin (OripAM.alpha.1) derived from the plasmid pAM.alpha.1, (d) a second DNA replication origin (Ori177) derived from the vector pACYC177, and (e) a polylinker region having recognition and cleavage sites for the restriction enzymes EcoRI at one terminal and HindIII at the other terminal of the polylinker DNA sequence.