The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 13, 1989

Filed:

Dec. 20, 1985
Applicant:
Inventors:

Keishin Sugahara, Kumamoto, JP;

Takayuki Imamura, Kumamoto, JP;

Fukusaburo Hamada, Nishigoshi, JP;

Nobuya Ohtomo, Kumamoto, JP;

Haruo Fujita, Kumamoto, JP;

Kazuhide Yagami, Kumamoto, JP;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P / ; G01N / ;
U.S. Cl.
CPC ...
435-7 ; 435-5 ; 435 68 ; 435235 ; 435239 ; 436820 ; 935 68 ; 935 69 ;
Abstract

A method for the purification of HBc antigen, particularly HBc antigen produced by recombinant organisms by means of DNA recombination technique, which comprises subjecting a solution containing HBc antigen to acid-treatment, wherein the solution containing HBc antigen is acidified to a pH range of not higher than 6 by adding an acid and then resulting precipitates of lipid and contaminant proteins are removed, and then subjecting the acid-treated solution containing HBc antigen to an ion exchange chromatography with an anion exchanger, and a method for measuring HBc antibody by using said purified HBc antigen in a passive hemagglutination method, EIA method or RIA method.


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