The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 30, 1989
Filed:
Sep. 12, 1988
Steven J Breiner, Durham, NC (US);
Charles H Lochmuller, Durham, NC (US);
Duke University, Durham, NC (US);
Abstract
An ampholyte separation apparatus and method which utilize a thermally engendered pH gradient to separate amphoteric molecules according to their isoelectric points and/or mobilities. Two independently temperature controllable thermal masses located on opposing ends of a plurality of barriers situated transverse to a flow channel create a pH gradient along the length of the transverse barriers. A pair of electrodes create an electric field along the length of the transverse barriers so as to cause ampholytes traveling down the flow channel to migrate into the transverse barriers and stabilize at points corresponding to their isoelectric points. Alteration of the pH gradient causes the separated amphoteric molecules to reenter the flow stream at different times and to be separately eluted and collected.