The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 15, 1988

Filed:

Apr. 19, 1984
Applicant:
Inventors:

David H Gelfand, Oakland, CA (US);

Frances C Lawyer, Oakland, CA (US);

Susanne Stoffel, El Cerrito, CA (US);

Assignee:

Cetus Corporation, Emeryville, CA (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P / ; C12N / ; C12N / ; C07H / ;
U.S. Cl.
CPC ...
435 68 ; 4351723 ; 435253 ; 435254 ; 435255 ; 435320 ; 536 27 ; 935 14 ; 935 27 ; 935 28 ; 935 29 ; 935 47 ;
Abstract

A novel universal dominant selectable marker cassette is disclosed. The marker comprises the coding sequences for aminoglycoside phosphotransferase I (APH-I) which has been modified and truncated so as to render its use in recombinant vectors more convenient. The modified, truncated sequence (mtAPH-I) gene is capable, upon expression, of conferring resistance to a number of antibiotics on the host. One of these antibiotics, G418, is toxic to eucaryotic as well as procaryotic hosts. Also disclosed are methods of constructing fusion proteins having N-terminal sequences corresponding to a desired peptide sequence, and C-terminal sequences comprising the amino acids encoded by mtAPH-I.


Find Patent Forward Citations

Loading…