The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 22, 1988
Filed:
Dec. 29, 1982
Applicant:
Inventors:
W Dorsey Stuart, Honolulu, HI (US);
Mark B Frank, Irvine, CA (US);
Assignee:
University of Hawaii, Honolulu, HI (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ; G01N / ; C07K / ;
U.S. Cl.
CPC ...
435-6 ; 435-7 ; 435 68 ; 4351722 ; 43524027 ; 435188 ; 435810 ; 436501 ; 436503 ; 436504 ; 436518 ; 436527 ; 436543 ; 436548 ; 436804 ; 436808 ; 530387 ; 530389 ; 530809 ; 935104 ; 935108 ; 935110 ;
Abstract
Monoclonal antibodies are provided capable of distinguishing DNA-RNA hybrid complexes from single stranded DNA and RNA and double stranded DNA and RNA. The antibodies find particular use in determining the presence of a specific nucleic acid sequence on a solid surface. Single stranded polynucleotide is fixed to a solid (gel) surface and then hybridized with the complementary probe. The hybrid complex specific monoclonal antibody is then added to bind to any hybrid complexes which have formed. By appropriate label, the hybrid complex may be visualized in a variety of ways.