The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 23, 1988
Filed:
Sep. 30, 1983
Robert F Santerre, Zionsville, IN (US);
Ramachandra N Rao, Indianapolis, IN (US);
Eli Lilly and Company, Indianapolis, IN (US);
Abstract
The present invention comprises novel recombinant DNA cloning and expression vectors which confer hygromycin B and/or G418 resistance to eukaryotic and prokaryotic host cells. The novel recombinant DNA vectors are derived from plasmid pKC203, a plasmid which can be isolated from E. coli JR225 (ATCC 31912). The hygromycin B and G418 resistance-conferring genes can be isolated on the 7.5 kb BglII restriction fragment or the 2.5 kb SalI-BglII restriction fragment of plasmid pKC203. The eukaryotic recombinant DNA vectors of the present invention are prepared by inserting such resistance-conferring restriction fragments into a vector, such as plasmid pSV5gpt, that comprises a eukaryotic promoter and the necessary functions for maintenance of the vector as an extrachromosomal element or as an integrated sequence in the host cell chromosomal DNA. Furthermore, the present invention comprises useful derivatives of plasmid pKC203 which, although comprising no eukaryotic elements, are useful recombinant vectors for E. coli and other prokaryotes and serve as starting material for the construction of eukaryotic vectors that confer hygromycin B and/or G418 resistance to eukaryotic host cells. One useful derivative of plasmid pKC203 is constructed by circularizing the .about.7.5 kb BglIII restriction fragment of plasmid pKC203 to form plasmid pSC701, which can be further digested with HaeII or SauIIIA1 to form smaller plasmids. The present invention also comprises the novel transformants of the aforementioned recombinant DNA vectors.