The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 08, 1987

Filed:

Dec. 24, 1984
Applicant:
Inventors:

David H Gelfand, Oakland, CA (US);

Frances C Lawyer, Oakland, CA (US);

Assignee:

Cetus Corporation, Emeryville, CA (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P / ; C12P / ; C12N / ; C12N / ; C12N / ; C07H / ;
U.S. Cl.
CPC ...
435 68 ; 4351723 ; 435253 ; 4353171 ; 435 91 ; 536 27 ; 935 11 ; 935 29 ; 935 41 ; 935 43 ; 935 45 ; 935 73 ;
Abstract

Two types of convenient portable control cassettes for the expression of protein encoding sequences in procaryotes are disclosed. Both cassettes comprise the P.sub.L promoter from lambda phage, which is controllable by means of a temperature sensitive repressor, operably linked to the ribosome binding site for N-gene (N.sub.RBS). In one embodiment, this cassette is bordered by restriction sites upstream of the P.sub.L promoter and immediately downstream from the N.sub.RBS permitting the insertion of a desired sequence containing its own start codon downstream from the cassette. The other embodiment contains an ATG start codon within the cassette and has a restriction site immediately 3' of the start codon.


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